Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTRRKPMKFMTLNTHSWLEPE-----PEKKLQELADKILLEDYEVIALQEINQLLE-SEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSH---------IGYDKYEEGNALLTKKTLES-QVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTD------KKSGFYFEWKNLENYFLE----TRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKAATVEKKID-----GWEQNTEKLRIDFIFVPEGMQVK-------------KYQRI--FDGIDSPIISDHYGVEIELDVNE 1ZWX Chain:A ((7-299)) ---YPGNFKITSHNVYLFSRNIYPNWGQMHRADLIAQADYMKNNDVVILNEAFDTSASHRLLN---------------------------NLRE-MYPHQTPVIGRSKHGWDKTEG---ALEDGGVAVVSQWPIVEKSQHIFQRGGGADRLSNKGFAYVKIMKNGKPYHIIGTHTQADDSLISKDTSRAIRAEQMQEIQTFIAKKNIPKDEIIFIGGDLNVNYGTDEYHDMLK---LLNVSSPA-NFNGQMATWDPTTNSMLKESYP-KAAPEYLDYIFVENGHARPHSWHNKVLHTKSPQWSVKSWFKTYTYQDFSDHYPVVGFTD---

General information: TITO was launched using: RESULT: Template: 1ZWX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1313 58697 44.70 242.55 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 44.70 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_1ZWX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ZWX-query.scw PDB file : Tito_Scwrl_1ZWX.pdb: