Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MINMEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTTFSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQINASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL 5WTQ Chain:B ((15-123)) ----VSLHNFSARLWEQLVHFHVMRLTDSLFLWVGA-T-PHLRNLAVAMS-----I--PVSTSLLGDTSDTTSTGLAQRLARKTNKQVFVSYNLQNTDSN------FALLVE---NRIKEEMEAFP----------------

General information: TITO was launched using: RESULT: Template: 5WTQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 387 -34316 -88.67 -329.96 target 2D structure prediction score : 0.46 Monomeric hydrophicity matching model chain B : 0.60 3D Compatibility (PKB) : -88.67 2D Compatibility (Sec. Struct. Predict.) : 0.46 1D Compatibility (Hydrophobicity) : 0.60 QMean score : 0.202

(partial model without unconserved sides chains): PDB file : Tito_5WTQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5WTQ-query.scw PDB file : Tito_Scwrl_5WTQ.pdb: