Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------MIKKMIYFL---GSLITVLS-----SFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQKQQLQITSDYTFLINGQSLG-TSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYD-ESADTSYKLLSEK------------- 1VCB Chain:C ((63-204)) LRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQE

General information: TITO was launched using: RESULT: Template: 1VCB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 453 8201 18.10 72.58 target 2D structure prediction score : 0.42 Monomeric hydrophicity matching model chain C : 0.63 3D Compatibility (PKB) : 18.10 2D Compatibility (Sec. Struct. Predict.) : 0.42 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.245

(partial model without unconserved sides chains): PDB file : Tito_1VCB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VCB-query.scw PDB file : Tito_Scwrl_1VCB.pdb: