Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPFHSTPNKGGTFDMTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQLLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFYTSEEKAKAHLDAGVKRVVISAPAGA-MKTIVYNVNDDTLDENDKIISAGSCTTNCLAPMAYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAIGLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYDDRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFANL 5VMT Chain:D ((9-339)) --------------MSIKVAINGFGRIGRLALRQI-EKAHGIEVAAVNDLTPAEMLLHLFKYDSTQGRFQGTAELKDDAIVVNGREIKVFANPNPEELPWG-ELGVDVVLECTGFFTNKTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMAAVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIGLVIPELNGKLDGSAQRVPVATGSLTELVSVLERPATKEEINAAMKAASSE--SYGYNEDQIVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFA--

General information: TITO was launched using: RESULT: Template: 5VMT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1982 -86915 -43.85 -263.38 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.88 3D Compatibility (PKB) : -43.85 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.88 QMean score : 0.521

(partial model without unconserved sides chains): PDB file : Tito_5VMT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5VMT-query.scw PDB file : Tito_Scwrl_5VMT.pdb: