Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDVRKSLQTILLKEAISMKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKVTASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSARLDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDTLLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQLGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANGNVTLKTWD-SNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNK----RVRADLVFYLPKRVYDHAAIKLLNGNIMIE-TLEAKDIYTKST-NGNIIVDQ-LTATMLEVEG-VNGNIDIRNGNILDSIIET-VNGTVTFG-ATAENLSVSLV-NGDVRLTIKEDNLKKVEAS-SVNGNVKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQLSTTTGSIYLKDTDK 4OPW Chain:A ((17-223)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TIDDFNAIKFDG-VIDFNYEQSESTPHIEITVDEN------------LHPYVNIDIQDRVLTVGFKGAKVDHFTKFIVKTNSKW--LKEVKAS-GNANFIANSPLKGDELKINANSNCLVQLKQKVEVGKLDLNVSGSANMVVNELKTDKLECSINGSGTINLKAGNAEEADYSITTDGEIMAFGVA--VPEVNCKITGKGSAQIHPTDN--LKATI-VGKGNIRYKG----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4OPW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1149 50105 43.61 258.27 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 43.61 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_4OPW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4OPW-query.scw PDB file : Tito_Scwrl_4OPW.pdb: