Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTIIYKRWLFMKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEVEPDLILLDLMLPKMDGLEVAREVRK-TYDMPIIMVTAKDSEIDKVLGLELGADDYVTKPFSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHREFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYLVTRRGVGYYLRNPEQE 5ED4 Chain:B ((25-249)) ------------RVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRPDAVILDVMMPGMDGFGVLRRLRADGIDAPALFLTAR-----KIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG-----------VRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGDVNVVESYVSYLRRKID--TGEKRLLHTLRGVGYVLREP---

General information: TITO was launched using: RESULT: Template: 5ED4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1011 -60364 -59.71 -286.08 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -59.71 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.567

(partial model without unconserved sides chains): PDB file : Tito_5ED4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ED4-query.scw PDB file : Tito_Scwrl_5ED4.pdb: