Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIGRNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVTQPIIQALHELGVAGAELKGRNDLVIDGM----KFSGNAMYATNGRMFAHGTIMFDSDINEVVNALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEVKTYELTDEDWKKINRISEEYYR--NWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMIKEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY 4TVY Chain:B ((12-306)) -----SDSYDPWFNLAVAECIFRQMPATQRVLFLWRNADTVVIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMA---GKPEYDKTISTSIVLNALNALGVS-AEASGRNDLVVKTVEGDRKVSGSAYRETKDRGAHGGTLLLNADLSRLANYLN----------------RVTNLTELLPG-----ITHEQVCEAITEAFFAHYGERVEAEIISPNKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHITRAQVFTDSLNPAPLEALAGRLQGCLY-------------------------------

General information: TITO was launched using: RESULT: Template: 4TVY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1370 -57667 -42.09 -211.23 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.76 3D Compatibility (PKB) : -42.09 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.471

(partial model without unconserved sides chains): PDB file : Tito_4TVY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TVY-query.scw PDB file : Tito_Scwrl_4TVY.pdb: