Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHEPADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLEDSVLIGPRVNLITVNHLTDPKER-RGLS-VKPIHIKKNAWIGAGATILPGVTIGENAIVAANATVTKDVPDNTIVAGIPAKIVKPVERII 3V4E Chain:B ((20-188)) ----FDQYLINERARAKDICFELNHT------RPSAT---------NKRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVFIGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAGSVVTKDIPPHSLAVGNPCKVVRKID---

General information: TITO was launched using: RESULT: Template: 3V4E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 844 -67108 -79.51 -401.84 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.80 3D Compatibility (PKB) : -79.51 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.588

(partial model without unconserved sides chains): PDB file : Tito_3V4E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3V4E-query.scw PDB file : Tito_Scwrl_3V4E.pdb: