Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGIGIAAPQVGQNKRIAVIEV--DEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRADEVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEHLDD 4WXK Chain:D ((6-145)) ----VLIYPDDHLKVVCEPVTEVNDAIRKIVDDMFDTMYQEKGIGLAAPQVDILQRIITIDVEGDKQNQFVLINPEILASEGETGIEEGCLSIPGFRALVPRKEKVTVRALDRDGKEFTLDADGLLAICIQHEIDHLNGILFVD---------------------

General information: TITO was launched using: RESULT: Template: 4WXK.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 695 -23667 -34.05 -171.50 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -34.05 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_4WXK.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4WXK-query.scw PDB file : Tito_Scwrl_4WXK.pdb: