Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------MTHKIGVVGD-----KDSVLPFKLFG------FDVRYGTTKQTVRQAIEEMAKNEY--GVIYVT----EQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQK---NVEKAVGQNIL-------- 2SCU Chain:B ((245-385)) AAQWELNYVALDGNIGCMVNGAGLAMGTMDIVKLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIGAVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAV

General information: TITO was launched using: RESULT: Template: 2SCU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 512 -19685 -38.45 -191.11 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.63 3D Compatibility (PKB) : -38.45 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.425

(partial model without unconserved sides chains): PDB file : Tito_2SCU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2SCU-query.scw PDB file : Tito_Scwrl_2SCU.pdb: