Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNLSNRFSPRLSRIEVSKIRQFDQQISSI----PDVIKLTLGEPDFPTPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGATEAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERAFEQYGSAV----KAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTHMSIATYLP--EQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQKAAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTDSWQFCVELAEQAKVACIPGIAF-GPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ 1O4S Chain:B ((15-388)) --------SRRISEIPISKTMELDAKAKALIKKGEDVINLTAGEPDFPTPEPVVEEAVRFLQKGEVKYTDPRGIYELREGIAKRIGERYKKDISPD-QVVVTNGAKQALFNAFMALLDPGDEVIVFSPVWVSYIPQIILAGGTVNVVET------FMSKNFQPSLEEVEGLLVGKTKAVLINSPNNPTGVVYRREFLEGLVRLAKKRNFYIISDEVYDSLVYTDEFTSILDVSEGFDRIVYINGFSKSHSMTGWRVGYLISSEKVATAVSKIQSHTTSCINTVAQYAALKAL---EVDNSYMVQTFKERKNFVVERLKKMGVKFVEPEGAFYLFFK----VRGDDVKFCERLLEEKKVALVPGSAFLKP---GFVRLSFATSIERLTEALDRIEDFLNS-

General information: TITO was launched using: RESULT: Template: 1O4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2016 -3558 -1.76 -9.80 target 2D structure prediction score : 0.73 Monomeric hydrophicity matching model chain B : 0.79 3D Compatibility (PKB) : -1.76 2D Compatibility (Sec. Struct. Predict.) : 0.73 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.592

(partial model without unconserved sides chains): PDB file : Tito_1O4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1O4S-query.scw PDB file : Tito_Scwrl_1O4S.pdb: