Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRIVYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEYTIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESSTENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMSSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK 4MQV Chain:B ((5-30)) -------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LTEEQRKKIEENRQKALARRAEKLLA---------------------------

General information: TITO was launched using: RESULT: Template: 4MQV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 7 -77 -10.93 -2.94 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : -10.93 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.547

(partial model without unconserved sides chains): PDB file : Tito_4MQV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MQV-query.scw PDB file : Tito_Scwrl_4MQV.pdb: