Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSITVNILYTSTNG--QARKFAEEMVERNIVKAIRAEEGNEKYDYYFPL--------EDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP 1TUV Chain:A ((1-102)) -MLTVIAEIRTRPGQHHRQAVLDQFAK--IVPTVLKEEGCHGYAPMVDCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP--

General information: TITO was launched using: RESULT: Template: 1TUV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 315 20233 64.23 219.92 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 64.23 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.550

(partial model without unconserved sides chains): PDB file : Tito_1TUV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TUV-query.scw PDB file : Tito_Scwrl_1TUV.pdb: