Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTASLSSSPVRHPSVDNAAIPAASHHIPPPLNGYAFLEHRRSALSHDTFIARDVRQQQHPEQQDNDQAKVIIRVYALEYLRR-DEECRFALERECLAARLVVHPHLLPLGSPFASKTDLFVVEKYCAGGDLYELMVSLAKEGPIASETGAAKGEAPPRSSTGLPISTVKRFMRELLSAVQYLHRTCGLVHRNIKLETLFIDEE---QHLRLGSFGLCAVLPPPSVATGDREGALDAPEDKASSSHAPLRLCCGSKHYAAPELVQGHPYQGEGVDAWACGVVLFALLTGCFPFDSDDGDEALFRLLCGDVESHLAQHPAMAAIEDPQACDLVRNLLRPNPNARYTVSEALEHPYLWEV 3C0G Chain:B ((21-292)) ----------------------------LFEDVYELCEVIGKGPFSVVRRCINRE----------TGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLKHPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRAD------------------AGFVYSEAVASHYMRQILEALRYCHDN-NIIHRDVKPHCVLLASKENSAPVKLGGFGVAIQLGESGL---------------------VAGGRVGTPHFMAPEVVKREPY-GKPVDVWGCGVILFILLSGCLPFYGTKER--LFEGII-KGK--YKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKE-

General information: TITO was launched using: RESULT: Template: 3C0G.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1294 -91812 -70.95 -342.58 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -70.95 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.412

(partial model without unconserved sides chains): PDB file : Tito_3C0G.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C0G-query.scw PDB file : Tito_Scwrl_3C0G.pdb: