Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RGSYLTVKPSELDKHI--AQLEEYLRNPPCKVTIYAPPP---KKGRTQLRTEMG-----FASAGKNEDVPVKIAST---GHTYQCRTDATCEEPV-CHGLPNGWTYDGVYMHTFTELSLEQLKAQKAAQTSRH----------- 2JHB Chain:A ((1-143)) -GSMPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEFDEERAQQEDALAQQ

General information: TITO was launched using: RESULT: Template: 2JHB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 463 29506 63.73 250.05 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 63.73 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.195

(partial model without unconserved sides chains): PDB file : Tito_2JHB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JHB-query.scw PDB file : Tito_Scwrl_2JHB.pdb: