Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence PVNLQEVRSNDQQLHPYRCKFEVLHIWYTEPFLSSFIDPGQRAEITIEARARKTDFAIDIDGE---CKGTKVKGAGRIPE--NWFIRLSREGSKEVQFINDKPKNNRVSGGS 5HJ1 Chain:A ((4-100)) PLV-KRLREQPQNILTY---LSISPVLSGDKLLGYRLNPGKDASLFRQSGLQANDLAIALNGIDLRDQEQAQQALQNLADMTEITLTVEREGQRHDIAFAL-----------

General information: TITO was launched using: RESULT: Template: 5HJ1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 328 -1863 -5.68 -20.24 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -5.68 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_5HJ1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5HJ1-query.scw PDB file : Tito_Scwrl_5HJ1.pdb: