Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYVLLVPVNAEEDEDGQVE-----------------------------------------------IQAYSFIENEDGTE-GELQPIPEDSEDEWNMIEEVFNSFMEE 1VK9 Chain:A ((2-148)) GSDKIHHHHHHVEKNLLRSALKIFEKKDLS--LLAYSGRSIFESKDSGLKPVVELFKRFDNLEGSLVIDKMVGKAAASFLLKMKPDHIHAKVISKPALKLMNEYGQSFSYDEKIPFVLGKDGKSMCPFEKLVLEMDDPEEIIRIVLSKF---

General information: TITO was launched using: RESULT: Template: 1VK9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 389 54850 141.00 571.35 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 141.00 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.333

(partial model without unconserved sides chains): PDB file : Tito_1VK9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VK9-query.scw PDB file : Tito_Scwrl_1VK9.pdb: