Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQVELIQALPKAELHVHIEGTFEPELMFAIAQRNQIQIPYKSVEEVKQA-YNFHNLQSFLDIYYAGANVLVHEQDFYDLAWAYFEKCAEDRVVHTEMFFDPQTHT-DRGIAFATVINGLKRACADAKDKFGISSQLIMCFLRHLSEEAAFETLEQALPFK-EDIIAVGLDSSEVGHPPAKFERVFAKAREEGFLIVAHAGEEGP-PEYVWEALDLLKVNRIDHGVRSEEDEQLMARLINEKMPLTVCPLSNLKLCVVKD---MKDHNIRRLLQKGVHVTVNSDDPSYFGGYMNDNFFAIQQALDLTNDELKQLAINSFEASFISDEEKQKWISEIQKI 4GXW Chain:B ((21-354)) -AHRAFFHALPKVELHCHLLGAVRHDTFVALAQRSGAPIERAEIDAFYARGEKPVGVLHVLRALDRY--LLTRPDDLRRIAYEYLEDAAAHNVRHAEFFWNPTGTVRVSGIPYADAQAAIVTGMRDAARDFGIGARLIPSIDREQDPDEAVAIVDWMKANRADEVAGIGIDYRENDRPPELFWKAYRDARAAGFRTTAHAGEFGMPWRNVETAVDLLHVDRVDHGYTIVDNPELCARYAERGIVFTVVPTNSYYLRTLPPDQWAERHPMRKMPGLGLKIHPNTDDPTLHKVNPSEAWELMFSHFGFTIADLKQFMLNGIDGAWVDDDTKAAWRAAWA--

General information: TITO was launched using: RESULT: Template: 4GXW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1921 1478 0.77 4.52 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 0.77 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_4GXW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GXW-query.scw PDB file : Tito_Scwrl_4GXW.pdb: