Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIPKSFLIYKNDKLDLCKTLAIIEERLELPSPMEQDDMAGKTLYDKLWDDHVVT--QRDDGSCLLYIDRHLLHEVTSPQAFEGLQLAGRQPWRLSANVATPDHNVPTSKKERDQGIAGIEDDTSRIQVQTLDDNCKAFNIVEFGINDIRQGIVHVVGPEQGLTLPGMTVVCGDSHTATHGAFGCLAHGIGTSEVEHVLATQCLVQKKSKNMLVRVDGVLGKGVTPKDVVLAI----I--GKIGT------AGGTGYAIEFGGQVFRDMSIEGRMTVCNMAIEAGAR-VGMVAVDDKTIEYVKGR----------SYAPKG------EQWDQAVAYWNTLHSDDDAVFDAVVELNGAEI-EPQVSWGTSPEMVIPVSKAVPTLEQAKDDVQRNDWTRAYQYMGLNAGQALADIQLGRVFIGSCTNSRIEDIRAAAEVVKGRKVAPSIKQAMIVPGSGLVKQQAEKEGLDKIFLEAGFEWREPGCSMCLAMNADKLQPGEHCASTSNRNFEGRQGNGGRTHLVSPAMAVAAAIAGHFVDIRSF 1L5J Chain:B ((356-826)) --------------KAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRACGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVD------------VNTHHTLPDFIMNRGGVSLR---PGDGVIHSWLNR--MLLPDTVGTGGDSHTRFPI---GISFPAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVEKKGKKNIFSGRILEIEG--LPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNIVLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPILCAPNDPDDARPLSA-------------------------------VQGEKIDEVFIGSCMT-NIGHFRAAGKLLDAHKGQLPTR-LWVAPPTRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGN-QARVADGATVVSTSTRNFPNRLGTGANVFLASAELAAVAALIGKLPTPEEY

General information: TITO was launched using: RESULT: Template: 1L5J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2433 403 0.17 0.92 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : 0.17 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.388

(partial model without unconserved sides chains): PDB file : Tito_1L5J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1L5J-query.scw PDB file : Tito_Scwrl_1L5J.pdb: