Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLFDDTAVSKQLAQVRIVMVNTTLPANIGSALRAMKTMGLSKLVLVSPKTYPHPDIQALAAGAQDLLEQVEIVETLEEAIKDCHLVFGTSARSRTIPWPLLDVRPASKEAIKAAAQGQQIAIVFGREDRGLTNEELALANYHLTIPVNSDYGVLNVAQAIQVVCYELRMSVLEQKEVASDAGQMPLAQGQSMQWDEPLVTQQQMEEFYPHLEKVLTEIEFLDPENPRLLPLRLRRLFGRIQLDRMEYHLLRGIFSRVQALTSGKWKKASSDKEDQSNA 5GM8 Chain:C ((5-168)) ---------SMMLDRIRVVLVNTSHPGNIGGAARAMKNMGLSQLVLVQPESFPHGDAVARASGATDILDAARVVDTLEEALSGCSVVLGTSARDRRIPWPLLDPRECATTCLEHLEANGEVALVFGREYAGLTNEELQRCQFHVHIPSDPEFGSLNLAAAVQVLTYEVRMAWL----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5GM8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 883 -148832 -168.55 -907.51 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain C : 0.74 3D Compatibility (PKB) : -168.55 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_5GM8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5GM8-query.scw PDB file : Tito_Scwrl_5GM8.pdb: