Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVKNSPAELSSALVNQRNDVPLIRYSGRFAPSPTGPLHFGSLITAVASYCDARAHQGRWLVRVEDTDIPRIYPGSEDHILASLE--AFQFDPDAEI------IFQKDRLDIYESVLDQLKKEGLVYACQCTRKML----GSNAIYAGTCRDLQLDFQHQ--------AIRVKV-QDQPICFDDRLQGLHCSNLEHDLGDFVLKRRDGIINYQLAVVADDYLQGITHVVRGADLLDNTERQIWLGQLLGYPKLSYMHLPLAMNDQGQKLSKQNLAHALDLTKAPELLQQAIQALGQPQVDLARPEVMLKQAVTQWNVDLIPHGQQLCGTYL 1N78 Chain:B ((5-267)) ----------------------------RIAPSPTGDPHVGTAYIALFNYAWARRNGGRFIVRIEDTDRARYVPGAEERILAALKWLGLSYDEGPDVGGPHGPYRQSERLPLYQKYAEELLKRGWAYRAFETPEELEQIRKEKGGYDGRARNIPPEEAEERARRGEPHVIRLKVPRPGTTEVKDELRGVVVYD-NQEIPDVVLLKSDGYPTYHLANVVDDHLMGVTDVIRAEEWLVSTPIHVLLYRAFGWEAPRFYHMPLLRNPDKTKISKRKSHTSLDWYKAEGFLPEALR---------------------------------------

General information: TITO was launched using: RESULT: Template: 1N78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1126 10009 8.89 41.36 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain B : 0.74 3D Compatibility (PKB) : 8.89 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.74 QMean score : 0.452

(partial model without unconserved sides chains): PDB file : Tito_1N78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1N78-query.scw PDB file : Tito_Scwrl_1N78.pdb: