Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLHCCFTTMPLNDNFVYAPPQDPLSILFEDDDLIVVDKPAGLLSVMGRLPEHHDSAYLRVLEKFPLAKVTHRLDMATSGLLMFAKHRDAEVAVSKMFQARTVKKHYIALVQGQVK-QEGSVEVPLITDWENRPRQIVHFELGKHAKTLFQPLVYDATTDQSRVLLEPVTGRSHQLRVHMMHIGHPIMGDKLYHPEPKRFHLNRMALHAAYLAFQHPLKGTDVVIESQVPF 2I82 Chain:D ((7-217)) ------------------PQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKDSVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWGHPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAA-DNTARVVLKPITGRSHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPADF

General information: TITO was launched using: RESULT: Template: 2I82.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1096 -62971 -57.45 -299.86 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain D : 0.79 3D Compatibility (PKB) : -57.45 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_2I82.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I82-query.scw PDB file : Tito_Scwrl_2I82.pdb: