Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSLVVPAEHPETVHNETDRVERILVVDDDVRLRTLLQRFLEDKGFVVKTAHDASQ-MDRLLQRELFSLIVLDFMLPVEDGLSICRRLRQSNIDTPIIMLTARGSDSDRIAGLEAGADDYLPKPFNPNELLARIRAVLRRQVREVPGAPSQQVEVVSFGPWSLDLSTRTLTREGQIVTLTTGEFAVLKALVQHPREPLTRDKLMNLARGREWGAMERSIDVQVSRLRRLIEDNPARARYIQTVWGVGYVFVPDGAE 5ED4 Chain:B ((25-246)) ---------------------RVLVVDDEANIVELLSVSLKFQGFEVYTATNGAQALDRARETRP-DAVILDVMMPGMDGFGVLRRLRADGIDAPALFLTAR-----KIAGLTLGGDDYVTKPFSLEEVVARLRVILRRAG-----------VRLTFADIELDEETHEVWKAGQPVSLSPTEFTLLRYFVINAGTVLSKPKILDHVWRYDFGGDVNVVESYVSYLRRKI--DTGEKRLLHTLRGVGYVL------

General information: TITO was launched using: RESULT: Template: 5ED4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 978 -49870 -50.99 -239.76 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : -50.99 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.477

(partial model without unconserved sides chains): PDB file : Tito_5ED4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5ED4-query.scw PDB file : Tito_Scwrl_5ED4.pdb: