Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDILENPLELCGFAFIEFVSKE-NELDPIFETIGFSKVAKHKSKKAYLWRQGNINIILNYQPESYASFFFNEHGPSACAMGFKTRDAAKAFKKAVELGAEPMYSKVGPMELNIPAIKGIGGMPIFLVDCDIY-----ENDFVFFDDAQRNPEGAGLKEIDHLTHNVYKGR--MEYWANFYEKIFNFQEIRYFDIKGEYTGLTSKALTAPDGMIRIPLNEDSDKGNGQIAEFLADFNGEGIQHIAFICDDLISTWDKLKGLGLKFMTPPPNTYYEMLEERLPEHGEPTEELKQRCILLDGSTKDGQKKLLLQIFSENMIGPVFFEFIQRKDDDGFGEGNFKALFESIERDQIRRGVLEAK 1CJX Chain:D ((4-355)) ---YENPMGLMGFEFIEFASPTPGTLEPIFEIMGFTKVATHRSKNVHLYRQGEINLILNNEPNSIASYFAAEHGPSVCGMAFRVKDSQKAYNRALELGAQPIHIDTGPMELNLPAIKGIGGAPLYLIDRFGEGSSIYDIDFVYLEGVERNPVGAGLKVIDHLTHNVY--RGRMVYWANFYEKLFNFREARYFDIKGEYTGLTSKAMSAPDGMIRIPLNEESSKGAGQIEEFLMQFNGEGIQHVAFLTDDLVKTWDALKKIGMRFMTAPPDTYYEMLEGRLPDHGEPVDQLQARGILLDGSSVEGDKRLLLQIFSETLMGPVFFEFIQRKGDDGFGEGNFKALFESIERDQVRRGVLA--

General information: TITO was launched using: RESULT: Template: 1CJX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1948 29383 15.08 85.42 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.90 3D Compatibility (PKB) : 15.08 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.472

(partial model without unconserved sides chains): PDB file : Tito_1CJX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1CJX-query.scw PDB file : Tito_Scwrl_1CJX.pdb: