Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MDFNCDLVWMNSMSALLRFTQFVQKTFALWVIIFAALALWQPEFFVWLKAYIPWILGIIMLGMGMTMTVDDFKGVLQSPKAVLIGVVAQFVVMPGLAF--ILCKL--FNLPPEIAVGVILVGCCPGGTASNVI---TYMAKGNVALSVACTSVSTLLAPVLTPAIFY--------------------LLASQWLKIDAASMFISILQVVLLPIVIGLILRTWLKRQVESYIQVMPLV-------------SVIAIVAIVAAIIGGSKAAILQSGLLILAVVILHNGLGYLLGFAAARFFK--LPYADSKAIAVEVGMQNSGLGV-ALAAVHFAASPITAVPSAIFSLWHNISGPALATYWASKHKQE 4XNV Chain:A ((38-382)) -SFKCALTKTG-----FQFYYLPAVYILVFIIGFLGNSVAIWMFVFHMK---PW--------SGISVYMFNLALADFLYVLTLPALIFYYFNKTDWIFGDAMCKLQRFIFHVNLYGSILFLTCISAHRYSGVVYPKSLGRLKKKNAICISVLVWLIVVVAISPILFYSGTGVRKNKTITCYDTTSDEYLRSYFIYSMCTTVAMFCVPLVLILGCYGLIVRALIYKMKKYTCTVCGYIYNPEDGDPDNGVNPGTDFKDIVCPLCGVGKDQFEEVE------EPLRRKSIYLVIIVLTVFAVSYIPFHVMKTMNLRARLDFQTPAMCAFNDRVYATYQVTRGLASLNSCVN----PILYFLAGDTFRRR

General information: TITO was launched using: RESULT: Template: 4XNV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 983 -163507 -166.33 -550.53 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -166.33 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.095

(partial model without unconserved sides chains): PDB file : Tito_4XNV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XNV-query.scw PDB file : Tito_Scwrl_4XNV.pdb: