Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSRAISHIDTFQGLILALQNYWAEQGCVVLQPYDMEM---------GAG--TFHTATFLRALGPETWNAAYVQPSRRPKDG-RYGENPNRLQHYYQFQVVLKPNPD----------NIQQLYLDSLKAIGIDTLTHDIRFVEDNWESPTLGAWGLGWEVWLNGMEVTQFTYFQQV---GG-V-----ECY---PVTGEITYGLERLAMYLQGVDSVYDLVWTKGQFGTVTYGDVFHQNEVEQSTYNFEYAPVDKLFELFDFYESEANRLMEAKLPLPAYEQVVKASHTFNLLDARGAISVTERQRYILRVRTLARAIAQSYVQARAELGFPMAEPHLRDEVLAQLKAQAESEAAKAEKN 2ZTG Chain:A ((56-267)) ----FKKPFELDEMREYYLNFFERRGHGRIERYPVVARWRTDIYLTIASIADFQPFVTSGVAPPPANPLTISQPCIRLDDLDSVGRT---GRHLTLFEMMAHHAFNYPGKEIYWKNETVAYCTELLNELGVKKE--DIVYKEEPWAG--GGNAGPCLEAIVGGLEVATLVFMNLEEHPEGDIEIKGARYRKMDNYIVDTGYGLERFVWASKGTPTVYDAIFPE----------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2ZTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 751 -12433 -16.55 -69.85 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -16.55 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.341

(partial model without unconserved sides chains): PDB file : Tito_2ZTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZTG-query.scw PDB file : Tito_Scwrl_2ZTG.pdb: