Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MISVGIVGGTGYTGVELLRILLRHP-----KAQVRVLTSRTEAGKPVADMFPNLRGHTDLQFSDLNIDALKECDVVFFATPHGVAMQHAKDLIAAGTKVIDLAADFRLQNLEQFEKWYGMEHACPDVLKDSVYGLTEL--NREKIKQAQVIGNPGCYPTTVQLGLAPLLKSAQALIETKNIIIDAKSGVSGAGRKASLGMIYSENADNFKAYGVAG-HRHHPEIVEALENIAGKKDVFEGLLFVPHLVPMIRGMLSTIYVDLTEAGKQTDLQALYENFYANEKFVDVMPANSSPETRSVRGANELRIAL-YKPQPNKLIILAAQDNLVKGASGQAVQNMNLMFGFNEDEGLQGIGLLP 2I3A Chain:C ((12-352)) ---VAVAGASGYAGGEILRLLLGHPAYADGRLRIGALTAATSAGSTLGEHHPHLTPLAHRVVEPTEAAVLGGHDAVFLALPHGHSAVLAQQL-SPETLIIDCGADFRLTDAAVWERFYGSSHA-----GSWPYGLPELPGARDQLRGTRRIAVPGCYPTAALLALF--PALAADLIEPAVTVV-AVSGTSGAGRAATTDLLGAEVIGSARAYNIAGVHRHTPEIAQGLRAVT-DRDV--SVSFTPVLIPASRGILATCTARTRSPLSQ--LRAAYEKAYHAEPFIYLMPEGQLPRTGAVIGSNAAHIAVAVDEDAQTFVAIAAIDNLVKGTAGAAVQSMNLALGWPETDGLSVVGVAP

General information: TITO was launched using: RESULT: Template: 2I3A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1983 -9564 -4.82 -28.81 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain C : 0.77 3D Compatibility (PKB) : -4.82 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.520

(partial model without unconserved sides chains): PDB file : Tito_2I3A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2I3A-query.scw PDB file : Tito_Scwrl_2I3A.pdb: