Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKLLSFLKNKALGSSIDYKILPRKVKFDWKDTPIDWIPNQ-PFASYFINEINNILPAGEFWFCRLYNKVLPRITDE----KLKQDVQAFIR--------QEAMHANA----HTSANKEYLSARNIDIQRNLDIMNYLFTTALADKPFDKEVPQFLQ----EQWDLFRLGVIAT-VEHMTCVLG-------KYALYNKRWEELGADPEMVD-LVKWHGSEEIEHRTVAFDLYRHLGGG-----------YIPRYYLSL--------------------------AVIVLVLG-LWV-DGAAHI-MKQDPRFADAA-----------KSRFFAAWVALEWY----------KISRKDNQVL-PNPIWLIAQQI---DYLMPWYD---------PVKEGSTEDAVSYL---SQSPAAKRAELQAA------------------------------- 5UAM Chain:A ((23-462)) -DRQVGYFADNGVGNPLAIVQHPAGIHKNGITYVSYQGPKEDPYIASYNHQTGQWQGPFRAGISELGRRDGGKKFDNHGKPTMLIDDEGYIHIFYGGHGGQASNGKNPLGNTHHGANKHAVSKRPYDISQWEDLNNI--------TPFG-TYNQAIKMDNGDIYLFFRHGAHRSDWVYQKSVDNGRTFASPVSFLKHKRRT----DIDAVDSWYAWAGKGQGDNIIVSYDYHVCWDGGAGVNGRGHTTERHDVYFMSFNTKTGEWSNVEGEKLVLPVTREVADEKTMAMRTGELWTFNGSTHLDAQGQPHIAINAGIDKGAKTGGPKQTRHVRWNGNEWVGGDKVIPQYERVSRGDFMVTDPENIRYLTTYNQDNDAVLSWWQSHDGGEHFVEDKTVLRKDNASFAISAFIKDAIPDAQMLVAEKVSDEGIKMYLVGEEGAVTRSLVDLKTAMP

General information: TITO was launched using: RESULT: Template: 5UAM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1817 18933 10.42 62.69 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 10.42 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.031

(partial model without unconserved sides chains): PDB file : Tito_5UAM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UAM-query.scw PDB file : Tito_Scwrl_5UAM.pdb: