Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQSRCNSIKDHLEFGSMNNMKYTYKPNYFFFAHKLVLFLKDYLLKHP---TEQNTTFNLQTIYDVFSHDLASSTTNLE-GILNIADE-YVLETEE------------GLLPLISSHSINLKNHVLSLEFSPKALTSLLSGRSLVNPKAA 2NRA Chain:C ((30-147)) ------------------------------LPAKRVMYMALALIDSKEPLERGRVFKIRAEDLAALAKITPSLAYRQLKEGGKLLGASKISLRGDDIIALAKELNSEELDLNIIEWIAYSPDEGYLSLKFTRTIEPYISS----------

General information: TITO was launched using: RESULT: Template: 2NRA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 249 -13646 -54.80 -146.73 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain C : 0.65 3D Compatibility (PKB) : -54.80 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.517

(partial model without unconserved sides chains): PDB file : Tito_2NRA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2NRA-query.scw PDB file : Tito_Scwrl_2NRA.pdb: