Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPV-SGAKVGGKIELDFASSDNDKSENVRIRHAYLTYN-----NWLFGQTTSNFLS-SHAPEMI-DFSTN-----IGGGTTRIPQVRYNYKLA----PATQLFVSAEEGNSSATG--------DSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSGA-------GDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFE----GKSYKDNRVGLMAKYSF 5FVN Chain:D ((5-342)) ----------------------------------------------------------------------------------------------NKDGNKLDLYGKVDG-LHYFSDDDS----------------QDGDQTYMRLGFKGETQVNDQLTGYGQWEYQIQGNSGENENNSWTRVAFAGLKFGDAGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNSDFFGLVDGLNFAVQYQGKNGSASGEDQTNNGRTELRQNGDGVGGSITYNLG-EGFGIGTA--VSSSKRTSSQNDLTYGNGDRAETYTGGLKYDAN-NIYLAAQYTQTYNATRVGN-----------LGWANKAQNFEVVAQYQFDFGLRPSVAYLQSKGKDLENGY----GDQDLLKYVDVGATYYFNKNMSTYVDYKINLLDDKEFTRNAGISTDDIVALGLVYQF

General information: TITO was launched using: RESULT: Template: 5FVN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1608 52289 32.52 173.14 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : 32.52 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_5FVN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FVN-query.scw PDB file : Tito_Scwrl_5FVN.pdb: