Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQTLIFMHETGKISIDFFKECIMQDIPMSRWLSPLMAFCLSFVIMATLAPTMGIQIDRQIDFWLLWFGTMLLLALPVCYLEIALAKRSKTTALNALSSLTREADSSPKWRVVGCLAVVFIPFLAGNVLSTASNILVAQFAPSISSQIIFVGLAVAALVLSFIPRQILILLMTLGVIASIVLANMMGSTLQPWHWTSVEFKEWGNATVLALVASGLGLGLYWQNSVGAVQAQEGATKTVLPIWLAQLIAVVAFGFFSLQAQLPVLTWIFTGVMTSALFVQLAREQLAQRQLMPVLQWVIIVVAIAVWAVP--EVHNLFTLILMQWGLLICLIYAVFAGWIMKISHLRKSMNFSNELFYNLWRIAVRIVLPLSIIVAMIAVIGQSI 4TQ3 Chain:A ((15-301)) SKYLRLLR-------------------PVAWLCFLLPYAVGFGFGITPNAS-----LQHAVLGLLSFAFWMAFSFTINALY------------------DRDVD-----RLHDGLNLSMQPLVTGEI------------SVREAWLYCIAFLALSLATAAAINEKFFLAMLGANIIGYVYSAPPR-------------FKAWPVMDVICNALAAV-LAFYAGLSIGGAE---------VPIAIYPAAFFLAATFYIPTAVSDYEFDKKAGLKNTPVFF---GPERALKSLYPLSAITVILWAYVFLMAERIEIKVISPLIIAYTLIYTFIINSRWDG---------EKLNVSPNLILTPFGIISALFIAYGFAVISV-------

General information: TITO was launched using: RESULT: Template: 4TQ3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1368 -280032 -204.70 -996.55 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -204.70 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.393

(partial model without unconserved sides chains): PDB file : Tito_4TQ3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4TQ3-query.scw PDB file : Tito_Scwrl_4TQ3.pdb: