Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----MKTFAPFFQVLGISITLCTQAVFADEGISTQEADSLIKDDIASTQ--------VLQEICPTFVGANKKLETNTQ------KIIAMYLSGYSNKSITFSALQNDSEYKTLLSEARQAAKEMDHHEQHELCEEVVNYKD--------------------------------------- 4JJ0 Chain:A ((55-235)) GGFVAPNVQFSEAHWQGMEALPLSIELKRKLKLPLDLEGLLIDETSLNAAVSGLLAGDVLVAINGRKVKTLKKMQKETRRVQMDRRASLTVYRKGRLLTLTLSEEKNLGLAQVETAPMILPGDIMPHPYRGPCTQCHAIGTTGHITPDPDGIVLPPGPIRAGAKMPHRDRGPCAACHAIIQ

General information: TITO was launched using: RESULT: Template: 4JJ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 547 28774 52.60 233.93 target 2D structure prediction score : 0.28 Monomeric hydrophicity matching model chain A : 0.58 3D Compatibility (PKB) : 52.60 2D Compatibility (Sec. Struct. Predict.) : 0.28 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.115

(partial model without unconserved sides chains): PDB file : Tito_4JJ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JJ0-query.scw PDB file : Tito_Scwrl_4JJ0.pdb: