Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPE-GENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKE-LVMLKQANAVAESFGYFFNEKGEVVYKV-PRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNA-PKQTWLITDEAAANEILKGVTL 2O0M Chain:A ((95-341)) ----------------------------------------------------------------------------------------------------------------HQIEKEMTQYFGIQRCIVVAGDSDIQKKVLSDFGDVLTNTLNLLLPNGEN-TIAVMGGTTMAMVAENMGSLETEKRHNLFVPARGGIGEAVSVQANSISAVMANKTGGNYRALYVPEQLSRETYNSLLQEPSIQEVLTLISHANCVVHSIGRALHMAARRKM-SDDEMVMLKQKNAVAESFGYFFDEEGKVVY-KIPRIGLQLKNLQEIPYVVAIAGGKTKAKAIRAYMKNAPKQT-WLITDEAAANEILK----

General information: TITO was launched using: RESULT: Template: 2O0M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1378 -27066 -19.64 -111.38 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -19.64 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.527

(partial model without unconserved sides chains): PDB file : Tito_2O0M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O0M-query.scw PDB file : Tito_Scwrl_2O0M.pdb: