Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISPALESTLDNRYNRKEFQKRGFLMIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRLLQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVSETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEISDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERV--DFATFSAHKFHGPRGTGFIYWKKGRRLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLS-LEIKEQKPEQTQRLKVALINALKTYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASSTLHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN 4EB5 Chain:B ((2-377)) ---------------------------AYFDYTSAKPVDERILEAMLPYMTESFGNPSSVHSYGFKAREAVQEAREKVAKLVNGGGGTVVFTSGATEANNLAIIGYAMRNARKGKHILVSAVEHMSVINPAKFLQKQGFEVEYIPVGKYGEVDVSFIDQKLRDDTILVSVQHANNEIGTIQPVEEISEVLAG--KAALHIDATASVGQIEVD---VEKIGADMLTISSNDIYGPKGVGALWIRKEAKLQPVILGGGQENGLRSGSENVPSIVGFGKAAEITAMEWREEAERLRRLRDRIIDNVLKI-EESYLNGHPEKRLPNNVNVRFSYIEGESIVLSLDMAGIQASTGSACSSKTLQPSHVLMACGLKHEEAHGTLLLTLGRYNTDEDVDRLLEVLPGVIERLRSMS

General information: TITO was launched using: RESULT: Template: 4EB5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2208 -96636 -43.77 -259.08 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -43.77 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.565

(partial model without unconserved sides chains): PDB file : Tito_4EB5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4EB5-query.scw PDB file : Tito_Scwrl_4EB5.pdb: