Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQGGNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLAGFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFEQEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIPRKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILLGTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNLISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL 4GUS Chain:C ((1-20)) ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ARTMQTARKSTGGKA----PRKQL---------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4GUS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 9 1390 154.44 69.50 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain C : 0.40 3D Compatibility (PKB) : 154.44 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.40 QMean score : 0.823

(partial model without unconserved sides chains): PDB file : Tito_4GUS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GUS-query.scw PDB file : Tito_Scwrl_4GUS.pdb: