Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDEEQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKNGKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLRPQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGMTQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQEKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIAEDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGLQDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPYNAN-SLISKAEKANYPMLEVRQ-GTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAILKTDNNG-IQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF 5M1K Chain:B ((33-190)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------PDHIYCIGADFGKNQDYSVFSVLDLDT-GA-IACLE-R---------------------------------MNGATWSDQVARLKALSED--YGHAYVVADTWGVGDAIAEELDAQGINYTPLPVKSSSVKEQLISNLALLMEKGQVAVPNDKTILDELRNFRYYRTASGNQVMRAY-GRGHDDIVMSLALAYSQYE-----------------------

General information: TITO was launched using: RESULT: Template: 5M1K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 710 -43157 -60.78 -278.43 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -60.78 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.418

(partial model without unconserved sides chains): PDB file : Tito_5M1K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5M1K-query.scw PDB file : Tito_Scwrl_5M1K.pdb: