Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RPYHGRIQDLRRPLLCDVYIEYPSGQVFDQALGHDIVPAGET----AEISG----FKCKT-VRGTCVILPTCDTPPEYRVTSWKFEREMSDPERTRFI----ARFPNVRI---- 2PIA Chain:? ((224-321)) -SFGATNTNARENTPFTVRLS-RSGTSFE-------IPANRSILEVLRDANVRVPSSCESGTCGSCKTA-LCSGEADHR------DMVLRDDEKGTQIMVCVSRAKSAELVLDL

General information: TITO was launched using: RESULT: Template: 2PIA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 244 5534 22.68 68.31 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 22.68 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_2PIA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2PIA-query.scw PDB file : Tito_Scwrl_2PIA.pdb: