Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNSLSEANTKFMFDLFQQFR-K-SKENNIFYSPISITSALGMVLLGAKDNTAQQISKVLHFDQVTENTTEKAATYHVDRSGNVHHQFQKLLTEF-NKSTDAYELKIANKLFGEKTYQFLQEYLDAIKKFYQTSVESTDFANAPEESRKKINSWVESQTNEKIKNLFPDGTIGNDTTLVLVNAIYFKGQWENKFKKENTKEEKFWPNKNTYKSVQMMRQYNSFNFALLEDVQAKVLEIPYKGKDLSMIVLLPNEIDGLQKLEEKLTAEKLMEWTSLQNMRETCVDLHLPRFKMEESYDLKDTLRTMGMVNIFNGD-ADLSGMTWS--HGLSVSKVLHKAFVEVTEEGVEAAAATAVVVVELSSPSTNEEFCCNHPFLFFIRQNKTNSILFYGRFSSP 1ATT Chain:A ((50-430)) -WELSKANSHFATAFYQHLADSKNNNDNIFLSPLSISTAFAMTKLGACNNTLTQLMEVFKFDTISEKTS-----------DQIHFFFAKLNCRLYRKANKSSELVSANRLFGDKSITFNETYQDISEVVYGAKLQPLDFKGNAEQSRLTINQWISNKTEGRITDVIPPQAINEFTVLVLVNTIYFKGLWKSKFSPENTRKELFYKADGESCSVLMMYQESKFRYRRVA-ESTQVLELPFKGDDITMVLILPKLEKTLAKVEQELTPDMLQEWLD--ELTETLLVVHMPRFRIEDSFSVKEQLQDMGLEDLFSPEKSRLPGIVAEGRSDLYVSDAFHKAFLEVNEEGSEAAASTVISIAGRS----RVTFKANRPFLVLIREVALNTIIFMGRVANP

General information: TITO was launched using: RESULT: Template: 1ATT.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2200 72660 33.03 195.85 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : 33.03 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.419

(partial model without unconserved sides chains): PDB file : Tito_1ATT.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1ATT-query.scw PDB file : Tito_Scwrl_1ATT.pdb: