Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSHTILLVQPTKRPEGRTYADYESVNECMEGVCKMYEEHLKRMNPNSPSITYDISQLFDFIDDL-ADLSCLVYRADTQTYQPYNKDWIKEKIYVLLRRQAQQAGK 1PU1 Chain:A ((1-91)) MSLRKL-----------TEGDLDEISSFLH---NTISDFILKRVSAKEIVDIDITVLVEYTDELKVDISAELYLDELSDADPGIVDEAVDAAYRSLESFLDGFRE

General information: TITO was launched using: RESULT: Template: 1PU1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 280 -13086 -46.74 -145.40 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : -46.74 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.348

(partial model without unconserved sides chains): PDB file : Tito_1PU1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1PU1-query.scw PDB file : Tito_Scwrl_1PU1.pdb: