Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKELYEEVQGTVYKCRNEYYLHLWELSDWDQEGMLCLHELISREEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEEVGEISHRISEGGLWLDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRDLRIVFKEFTIRTH 5IPM Chain:F ((90-165)) SRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDP--ERGFRFSTYATWWIRQTIERAIMNQTRTI---------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5IPM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain F - contact count / total energy / energy per contact / energy per residue : 238 -33101 -139.08 -435.53 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain F : 0.64 3D Compatibility (PKB) : -139.08 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.509

(partial model without unconserved sides chains): PDB file : Tito_5IPM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5IPM-query.scw PDB file : Tito_Scwrl_5IPM.pdb: