Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAYTERVFGNV-EGEDVLAYRFETD-GGYQLEVMTYGATILRYVAPDKAGNFANVILGFDDFDSYVG-NSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNASNCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEISYKV-TTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAK-TPEANRDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHSFALPAGH----DNAGFLYDQNSGRFLLFKTEAPCFVVYTANFVDES-VIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTFTSKTRYELVVK 4RNL Chain:B ((7-342)) --QVSREPFGTLDDGTRVDRWTLESGPAGLRVRVLTYGGIVQTVEAPDRDGMRGQLALGFADLASYAAHGGSYFGALVGRYANRIAGASFVLDGRTDALTPNNGRHSLHGGPGGFSRVVWDAREVD-GGVQLHRVSPDGEEGFPGALDVRVTYTLSA-GALRIVSCATT-DAPTVVNLTNHTYLNLGGDGSGSAAGHELRLAASRYTPVDGTGIPVPGAPAEVTGTRFDFRAARAVA-------------GAYDHNFALDGGVREAPRTVAELYDPRSGRALALATTEPGLQLYTADHLDGTLTGTSGVPYGPAAGLALETQHFPDSPNRPDFPSTVLRPGESYRSETVYAFSV-

General information: TITO was launched using: RESULT: Template: 4RNL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 2017 37393 18.54 114.70 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.77 3D Compatibility (PKB) : 18.54 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.463

(partial model without unconserved sides chains): PDB file : Tito_4RNL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4RNL-query.scw PDB file : Tito_Scwrl_4RNL.pdb: