Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVE--------------VSSLGVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRL-I---N-LPLLYLGTLMVGASVAVINVLLPSLIQANQP-KKIGFLTTLYVTSMGIATALASYLAVPIT--QASSWKGLILLLTLLCLATFLVWLPNHRYNHR------LAPQ------------------------------TKQKSQIKVMRNKQVWAIIIFSGFQSLI-----FYTVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVSLAGVIGISMLFF-----PI---NNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGLSQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL 5EQH Chain:A ((13-430)) -----MLAVGGAVLGSLQFGYNTGVINAPQKVIEEFYNQTWVHRYGESILPTTLTTLWSLSVAIFSVGGMIGSFSVGLFVNRFGRRNSMLMMNLLAFVSAVLMGFSKLGKSFEMLILGRFIIGVYCGLTTGFVPMYVGEVSPTALRGALGTLHQLGIVVGILIAQVFGLDSIMGNKDLWPLLLSIIFIPALLQCIVLPFCPESPRFLLINRNEENRAKSVLKKLRGTADVTHDLQEMKEESRQMMREKKVTILELFRSPAYRQPILIAVVLQLSQQLSGINAVFYYSTSIFEKAG--VQQPVYATIGSGIVNTAFTVVSLFVVERAG-RRT-LHLIGL-AGMAGCAILMTIALALLEQLPWMSYLSIVAIFGFVAFFEVGPGPIPWFIVAELF-SQGPRPAAIAVAGFSNWTSNFIVGMCFQYVEQLCG------------------------------

General information: TITO was launched using: RESULT: Template: 5EQH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1379 -147755 -107.15 -425.81 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -107.15 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.339

(partial model without unconserved sides chains): PDB file : Tito_5EQH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EQH-query.scw PDB file : Tito_Scwrl_5EQH.pdb: