Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MELVLPNNYVVIDEEEMMYLDGG--AIYIPRWAITGAITGAAYAALAAAGGGGLQLVLASYGLRSALVAGI-VKGLGVLGIHIGNAFANTVIRSIASAG----------IGAGADW----IFTNIIDGWDGRRDNQLRIG 5K8J Chain:B ((2-128)) --------AYVLDTNVAIHLRDGDPEVTTRVTALNGAILLSIISRVELEGGVYRE--AAQAGLRRSRLD-VMLKVLPVLDF--DGAAADEYRRIVESAGYSRRKVVDRMIAAQALAHRATFVTFNADDFRDIPGLSLLAW

General information: TITO was launched using: RESULT: Template: 5K8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 405 -24268 -59.92 -220.62 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -59.92 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.080

(partial model without unconserved sides chains): PDB file : Tito_5K8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5K8J-query.scw PDB file : Tito_Scwrl_5K8J.pdb: