Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTVLEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQALRNRT-PIETDPKLDTLAKFTLAVINTK-GRVGDEALSEFLEAGYTQQNALDVVFGVSLAILCNYANNLANTPINPELQPYA 2O4D Chain:A ((2-145)) -------------------------------TTRLEWAKASPDAYAAMLGLEKALAKAGLERPLIELVYLRTSQINGCAYCVNMHANDAR-KAGETEQRLQALCVWQETPYFTPRERAALAWTEQLARLSQGALPHGLLDELR-EHFDDKEIAELTLAVSAINAWNRFGVGMGMQPE-------

General information: TITO was launched using: RESULT: Template: 2O4D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 527 -37773 -71.68 -266.01 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -71.68 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.391

(partial model without unconserved sides chains): PDB file : Tito_2O4D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2O4D-query.scw PDB file : Tito_Scwrl_2O4D.pdb: