Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKMERNINMKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKTSAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAGDDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAFIDKEGKLVKTHPGFMEKDAILQTLKELS 2H1B Chain:D ((3-129)) ---------------------------------------------------------GSDAPNFVLEDTNGKRIELSDLKGKGVFLNFWGTWCEPCKKQFPYMANQYKHFKSQGVEIVAVNVG----ESKIAVHNFMKSYGV-NFPVVLDTDRQVLDAYDVSPLPTTFLINPEGKVVKVVTGTMTESMI--------

General information: TITO was launched using: RESULT: Template: 2H1B.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 585 -86764 -148.31 -683.18 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain D : 0.73 3D Compatibility (PKB) : -148.31 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.666

(partial model without unconserved sides chains): PDB file : Tito_2H1B.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2H1B-query.scw PDB file : Tito_Scwrl_2H1B.pdb: