Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIYFDNSATTKPYPEALETYMQVASKILGNPSSLHRLGDQATRILDASRQQIADLIGKKSDEIFFTSGGTEGDNWLIKGVAFEKAQFGKHIIVSAIEHPAVKESALWLKSQGFEVDFAPVDKKGLVDVEALAGLIRHDTILVSIMAVNNEIGSIQPIEAISEFLADKPTISFHVDAVQALAKIPTEKYLTERVDCATFSSHKFHGVRGVGFVYIKSGK--KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQM-KAVIRQALLNYPDIFVFSDEENFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGTLIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR 5USR Chain:C ((28-379)) -LYMDVQATTPLDPRVLDAMLPYLINYYG--------GWESEAAMERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVLDSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEIGRICSSR-KVYFHTDAAQAVGKIPLDVN-DMKIDLMSISGHKIYGPKGVGAIYIRRRPRVRVEALQ------------TVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALK--D----------------SYVLRAIGTDEDLAHSSIRFGI---------------------------

General information: TITO was launched using: RESULT: Template: 5USR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1702 -128218 -75.33 -410.95 target 2D structure prediction score : 0.60 Monomeric hydrophicity matching model chain C : 0.76 3D Compatibility (PKB) : -75.33 2D Compatibility (Sec. Struct. Predict.) : 0.60 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.506

(partial model without unconserved sides chains): PDB file : Tito_5USR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5USR-query.scw PDB file : Tito_Scwrl_5USR.pdb: