Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSVSLERELETRLVDRERVINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLLKEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLEFPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAMSSLGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKMQDVLFPSLLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPFTYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFKV 3EFG Chain:A ((8-58)) --------------------------------------------RDQELEARLVELETRLSFQEQALTELSEALADARLTGARNAELIRHLLEDL----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3EFG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2 -106 -53.00 -2.08 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.42 3D Compatibility (PKB) : -53.00 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.42 QMean score : 0.663

(partial model without unconserved sides chains): PDB file : Tito_3EFG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EFG-query.scw PDB file : Tito_Scwrl_3EFG.pdb: