Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTGRVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRIIQERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYKYQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDTDEEYR 5DED Chain:G ((12-204)) ---QWERFLVPYRQAVEELKVKLKGIRTLYEYEDDHSPIEFVTGRVKPVASILEKARRKSIPLHEIE-TMQDIAGLRIMCQFVDDIQIVKEMLFARKDFTVVDQRDYIAEHKESGYRSYHLVVLYPLQTVSGEKHVLVEIQIRTLAMNFWATIEHSL-YK----IPEKVKLRLQRASEAASRLDEEMSEIRGEVQEA--------------------------

General information: TITO was launched using: RESULT: Template: 5DED.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 740 -93416 -126.24 -496.89 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain G : 0.85 3D Compatibility (PKB) : -126.24 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.473

(partial model without unconserved sides chains): PDB file : Tito_5DED.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DED-query.scw PDB file : Tito_Scwrl_5DED.pdb: