Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTVEEEKAFLARHLKATEA-------GEFVTIDALFQAYKKELG--RSYTRDAFYQLLKRHGWRNITPRPEHPRKADAQTIVASKNKISIQEDKKAL 4XLG Chain:B ((16-75)) -------EFLTHLVKQEPDLLTRIYCFQPITMNDLINKLRNKDSFVDLIDDGTIREWTDKLGICIRS------------------------------

General information: TITO was launched using: RESULT: Template: 4XLG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 121 -4083 -33.74 -80.06 target 2D structure prediction score : 0.90 Monomeric hydrophicity matching model chain B : 0.66 3D Compatibility (PKB) : -33.74 2D Compatibility (Sec. Struct. Predict.) : 0.90 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.718

(partial model without unconserved sides chains): PDB file : Tito_4XLG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XLG-query.scw PDB file : Tito_Scwrl_4XLG.pdb: