Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKVLLGFMGAGKSTIAR----GLDPNYLDMDALIEKRLGMSIANFFAEKGEAAFRQVESEVLADL--LQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLYLRIVADKDNQRPLF--LNNSKEELVAIFQERQAWYEEVASRVLDVTKLSPEEIIEELR 3N2E Chain:B ((5-145)) ---VLIGFMGSGKSSLAQELGLALKLEVLDTDMIISERVGLSVREIFEELGEDNFRMFEKNLIDELKTLKTPHVISTGGGIVMHENLKGLGTT----FYLKMDFETLIKRLNQKERAKRPLLNNLTQAKE----LFEKRQALYEKNASFIID--------------

General information: TITO was launched using: RESULT: Template: 3N2E.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 493 -15772 -31.99 -118.59 target 2D structure prediction score : 0.50 Monomeric hydrophicity matching model chain B : 0.82 3D Compatibility (PKB) : -31.99 2D Compatibility (Sec. Struct. Predict.) : 0.50 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_3N2E.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3N2E-query.scw PDB file : Tito_Scwrl_3N2E.pdb: